Regulatory

Part:BBa_I7104

Designed by: Barry Canton   Group: iGEM04_MIT   (2005-04-11)


T7 promoter and sRBS

The T7 promoter and a specialized RBS form the regulatory region that can be used to express protein in a first generation Virtual machine. There should be no transcription or translation by host machinery. Protein should only be produced in the presence of T7 polymerase and ribosomes with a mutant 16s rRNA.

Usage and Biology

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

[edit]
Categories
//classic/regulatory/uncategorized
Parameters
None